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We supply a variety of fluorogens, each with their own spectral properties and binding affinities for particular FAPs.
For simplicity and clarity, we name the FAPs and fluorogens such that the alpha FAPs bind and activate alpha fluorogens, while the beta FAPs bind and activate beta fluorogens.
The names of our FAPs and fluorogens, and the references to them in the primary literature, are listed in the table below.
| SpectraGenetics name | Names used in primary literature |
|---|---|
| FAPs | |
| FAP alpha1 | MG13, HL4-MG (Szent-Gyorgyi et al 2008) |
| FAP alpha2 | dL5**, dNP138 (Szent-Gyorgyi et al 2012) |
| FAP beta1 | HL1.0.1-TO1, AMII.2 (Szent-Gyorgyi et al 2008) |
| FAP beta2 | HL1-TO1, scFv1 (Szent-Gyorgyi et al 2008) |
| Fluorogens | |
| αRED-np | MG-11p (Szent-Gyorgyi et al 2008) |
| αRED-p | MG-ester (Szent-Gyorgyi et al 2008) |
| αRED-pHC | Cy3(S/SA)pH (Perkins et al 2018) |
| αRED-MSSC | Unpublished |
| βGREEN-np | TO1-2p (Szent-Gyorgyi et al 2008) |
| βRED-np | TO1-2p-Cy5 (Fisher et al., 2012) |
Spectral and affinity data
| FAP | Kd for Fluorogens |
|---|---|
| Alpha1 | 3.2 nM for αRED-np and αRED-p |
| Alpha2 | <1 nM for αRED; 3 nM for αRED-pHC and αRED-MSSC |
| Beta1 | 3.1 nM for βGREEN; 13.5 nM for βRED |
| Beta2 | 360 nM for βGREEN |
| FLUOROGEN | Ex-Max | Em-Max |
|---|---|---|
| αRED-np | 640 nm | 670 nm |
| αRED-p | 640 nm | 670 nm |
| αRED-pHC | 560 and 640 nm | 670 nm |
| αRED-MSSC | 560 and 640 nm | 670 nm |
| βGREEN-np | 510 nm | 530 nm |
| βRED-np | 510 and 650 nm | 670 nm |
Fluorogens Product List
| Fluorogen | FAP | Features |
|---|---|---|
| αRED-np | FAPα2, FAPα1 | Membrane impermeant |
| αRED-p | FAPα2, FAPα1 | Membrane permeant |
| αRED-pHD | FAPα2, FAPα1 | Membrane impermeant; pH-sensitive FRET |
| αRED-SSC | FAPα2, FAPα2 | Membrane impermeant; reduction-sensitive FRET |
| βGREEN-np | FAPβ1 | Membrane impermeant |
| βRED-np | FAPβ1 | Membrane impermeant |
