GPCR Internalization and Trafficking Assays
...ion assays utilizing a cell line line expressing a FAP-tagged GPCR. 1. Add test compound plus red fluorogen 2. Allow time for response 3. Add green fluorogen 4. Read An example is shown in the images below: The left panel shows CXCR4 at the surface before the addition of agonist. The middle panel shows the image 20 minutes after the addition of agonist, clearly showing a significant response. The final panel shows the image after the addition of...
Read MoreLicensing
...SpectraGenetics standard user licenses are shown here. Reporter-tagged Full Length Genes FAP-tagged Genes To obtain additional use or commercial rights to any of our products please send an email to sales@spectragenetics.com...
Read MoreAntagonist Assay for GPCRs Using Flourogen Activating Peptides (FAPs)
...d (antagonist) allow time for response and add green fluorogen Read If the test compound acts as an antagonist, then all of the receptor will remain on the surface and it will all end up with the green fluorogen. If some of the receptor did internalize it would be red, as the green fluorogen would not be able to exchange with the red in the endosome. The images below show two examples of the results of increasing concentrations of antagonist using...
Read MoreReceptor Re-Sensitization Assay Using Fluorogen Activation Peptides (FAPs)
...low time for respone Add green fluorogen (optional – wash out agonist) Add test compound (resensitizer-changes rate of receptor re-sensitization) and allow time for response Read This assay allows for a number of interesting measurements including: Rate of receptor recycling (re-sensitization) as a function of agonist Rate of receptor recycling as a function of antagonist (with or without agonist present) Rate of receptor recycling as a function o...
Read Morefluorogen-exchange-gpcr-assays
...ion assays utilizing a cell line line expressing a FAP-tagged GPCR. 1. Add test compound plus red fluorogen 2. Allow time for response 3. Add green fluorogen 4. Read An example is shown in the images below: The left panel shows CXCR4 at the surface before the addition of agonist. The middle panel shows the image 20 minutes after the addition of agonist, clearly showing a significant response. The final panel shows the image after the addition of...
Read MoreGPCR Fluorogen Exchange Assays
...ion assays utilizing a cell line line expressing a FAP-tagged GPCR. 1. Add test compound plus red fluorogen 2. Allow time for response 3. Add green fluorogen 4. Read An example is shown in the images below: The left panel shows the chemokine receptor CXCR4 at the surface before the addition of agonist. The middle panel shows the image 20 minutes after the addition of agonist, clearly showing a significant response. The final panel shows the image...
Read MoreCustom Gene Tagging and Generation of Stable Cell lines
...These full length reporter tagged genes have the following advantages over commonly used cDNA constructs: Tagged proteins are produced at native levels. The full repertoire of splice-isoforms is produced. Tagged proteins exhibit native subcellular localization and native biological function SpectraGenetics can also generate stable cell lines expressing any of our full length genes. To discuss the construction of a new gene, tagging vector or cell...
Read MoreOrders
...tation form below. (If you wish to discuss your request prior to or during completion of the RFQ, you are encouraged to contact us at 1.412.488.9350.) 2. Complete Tagged Gene Order Form below upon receipt of Quotation Alternatively, you may complete the physical forms from the links beneath and either email them to admin@spectragenetics.com or fax them to 1.412.488.9355. Request for Quote Form Tagged Gene Order Form Request for Quotation Form Requ...
Read MoreContact
... We are located at 2403 Sidney Street, Pittsburgh, PA 15203 Phone: 412 488-9350 email: info@spectragenetics.com Please contact us by phone or email for more information about our products or to discuss your specific assay needs. ...
Read MoreServices
...n also screen compounds on our validated GPCR expressing cell lines. Contact us to discuss. To discuss the construction of a new GPCR, cell line or for screening compounds, please send an email to sales@spectragenetics.com and we will get back to you shortly....
Read MoreValidated GPCR Cell Lines for Drug Discovery
...SpectraGenetics has validated more than 100 cell lines expressing N-terminal FAP-tags. Cell Lines are ready for drug screening. Pricing: $7,950 per cell line Please contact sales@spectragenetics.com for further information. AVAILABLE CELL LINES ...
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