Full Length Reporter Tagged Genes
...for: Protein abundance and localization Transcriptional and translational regulation Post-translational modifications Protein-protein interactions Alternative splicing Knockdown of RNA and protein by RNAi Transcription factor binding sites (ChIP) SpectraGenetics’ tagged genes are based on end-sequenced fosmid clones, not cDNA. Each tagged clone carries the complete gene of interest embedded in approximately 40 kb of native human or mouse genomic...
Read MoreVectors for Expressing Fluorogen Activating Proteins (FAP-tags®)
...ations of cognate small-molecule fluorogens. The fluorescent FAP/fluorogen complex is called a fluoromodule. Neither the FAP nor the fluorogen is fluorescent by itself. FAP-tags® allow the user to turn the fluorescent signal on and off by adding or removing fluorogen, or to change the signal wavelength by substituting one fluorogen for another. Spectragenetics has developed two different types of FAPs, α and β. Experiments can be designed with bot...
Read MoreFluorogens and Empty Tagging Vectors
...ations of cognate small-molecule fluorogens. The fluorescent FAP/fluorogen complex is called a fluoromodule. Neither the FAP nor the fluorogen is fluorescent by itself. FAP-tags® allow the user to turn the fluorescent signal on and off by adding or removing fluorogen, or to change the signal wavelength by substituting one fluorogen for another. FAP-Tagging Kit for Live Cell Assays (manual) Vector Sequences and Annotation: pMFAPalpha2; pMFAPbeta1 S...
Read MoreGFP-tagged Mouse Embryonic Stem Cell Lines
...at expresses a fluorescent fusion protein that is controlled by the native regulation of the cell. The tag is placed using a novel system known as CD-tagging. This system places the DNA carrying the tag into an intron of a gene creating a new exon in the process. This exon then incorporates the tag into transcripts and ultimately into proteins. A schematic of the process is shown below: The fluorescence in each cell of a particular clonal line wil...
Read MoreCustom Gene Tagging and Generation of Stable Cell lines
...These full length reporter tagged genes have the following advantages over commonly used cDNA constructs: Tagged proteins are produced at native levels. The full repertoire of splice-isoforms is produced. Tagged proteins exhibit native subcellular localization and native biological function SpectraGenetics can also generate stable cell lines expressing any of our full length genes. To discuss the construction of a new gene, tagging vector or cell...
Read MoreLicensing
...SpectraGenetics standard user licenses are shown here. Reporter-tagged Full Length Genes FAP-tagged Genes To obtain additional use or commercial rights to any of our products please send an email to sales@spectragenetics.com...
Read MoreOrders
...tation form below. (If you wish to discuss your request prior to or during completion of the RFQ, you are encouraged to contact us at 1.412.488.9350.) 2. Complete Tagged Gene Order Form below upon receipt of Quotation Alternatively, you may complete the physical forms from the links beneath and either email them to admin@spectragenetics.com or fax them to 1.412.488.9355. Request for Quote Form Tagged Gene Order Form Request for Quotation Form Requ...
Read MoreContact
... We are located at 2403 Sidney Street, Pittsburgh, PA 15203 Phone: 412 488-9350 email: info@spectragenetics.com Please contact us by phone or email for more information about our products or to discuss your specific assay needs. ...
Read MoreServices
...n also screen compounds on our validated GPCR expressing cell lines. Contact us to discuss. To discuss the construction of a new GPCR, cell line or for screening compounds, please send an email to sales@spectragenetics.com and we will get back to you shortly....
Read MoreValidated GPCR Cell Lines for Drug Discovery
...SpectraGenetics has validated more than 100 cell lines expressing N-terminal FAP-tags. Cell Lines are ready for drug screening. Pricing: $7,950 per cell line Please contact sales@spectragenetics.com for further information. AVAILABLE CELL LINES ...
Read MoreVectors Expressing Human GPCRs
...Vectors that express human GPCRs with N-terminal FAP tags. SpectraGenetics offers a variety of vectors (pMFAP2, pBabe-FAP and pSH-FAP) for hundreds of human GPCRs. Pricing: $1,700 each Please contact sales@spectragenetics.com for further information AVAILABLE VECTORS ...
Read MoreTechnology
...applications can be viewed here. Please contact us at info@spectragenetics.com for more information about these assays, or to discuss your specific assay needs. Properties of our standard FAPs and fluorogens are summarized below A chart of our standard FAPs and fluorogens is shown below. FAP tags were developed at Carnegie Mellon University over the past decade and a half and are the subject of multiple patents including US8664364, US9023998, US92...
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